Print ISSN: 1681-6900

Online ISSN: 2412-0758

Author : Al-Salih, HanaaSaeed

Uptake of Zinc Nanoparticles by Prosopisfarcta L.Plants Callus Cultures

Rana Tariq Yahya; HanaaSaeed Al-Salih

Engineering and Technology Journal, 2014, Volume 32, Issue 3, Pages 615-622

The current study showed the ability of Prosopisfarcta L. plants to uptake and accumulate pollution caused metals present in the environment, and their role on the phytoremediation by indication of Zinc oxide nanoparticles uptake at various concentrations.
For this purpose Zinc oxide nanoparticles of 120 nm were used with concentrations of 1.0, 10, 50, 100µg ml-1.
Resultsof this study referred to the formation of Prosopisfarctaplants stems callus on MS modified medium supplemented with 1.0 mg L-1 NAA (Naphthalene acetic acid) and 4.0 mg L-1 TDZ (Thidizurone). It was clear from the results that callus cultures of Prosopisfarcta had the ability to uptake and accumulate nanoparticles of Zinc oxide.Scanning electron microscope (SEM) photography showed that 100µg ml-1of Zinc oxide nanoparticles was the higher to be accumulated by callus tissues ,photographs showed a high density of these particles on the surface of the cells. In the same time a linear increasing of callus fresh weight with the increasing of Zinc oxide nanoparticles concentration was recorded. The study revealed that Prosopis seedlings had the ability to uptake Zinc oxide at 50 µg ml-1more than the other concentrations.