Print ISSN: 1681-6900

Online ISSN: 2412-0758

Author : Abd Al-Kareem Hussain, Huda

Extraction, Identification and Antibacterial activity of Cyperus oil from Iraqi C. rotundus

Zeid abdul-Majid Nima; Majid Sakhi Jabier; Raghidah Ismaeel Wagi; Huda Abd Al-Kareem Hussain

Engineering and Technology Journal, 2008, Volume 26, Issue 10, Pages 1156-1163

Cyperus rotundus has many different uses and these were based on the different
parts of plant. The medical uses of cyperus have been used in medicine for
thousands of years. The parts of the cyperus used are its leaves, seeds and oil. The
Extraction process was carried out by steam distillation. Optimum organic
extractant determined. The collected oil was identified via Thin Layer
Chromatography (TLC) using a mixture of Ethylacetate: toluene (1:9) as
chromatographic eluent . This study was designed to extract and identify of
essential cyperus oil from C. rotundus .The Antibacterial activity of Cyperus oil
was studied for various microorganisms (Staphylococcus aureus , Klebsiella
pneumoniae, Proteus vulgaris, Streptococcus pyogenes , Eschirichia coli and
Pseudomonas aeruginosa ) using inhibition zone method (Aromatogram). The
MIC and MBC for each microbe were estimated. The oil of C. rotundus was shown
a remarkable activity against gram-positive bacteria, less antibacterial activity was
found against gram–negative bacteria and no activity was observed with the oil
against Pseudomonas aeruginosa and Proteus vulgaris. Novel method for
extraction and identification of chemical composition for Iraqi cyperus oil was
conducted. The study of the biological activities of this oil is very important
because of needing to be determined whether there is any correlation between the
biological activities and one or more of the chemical compounds purified from C.
rotundus oil.