Print ISSN: 1681-6900

Online ISSN: 2412-0758

Keywords : coli

Use of Nano-Magnetic Material for Removal of Heavy Metals from Wastewater

G.K. Salman; A.J. Bohan; G.M. Jaed

Engineering and Technology Journal, 2017, Volume 35, Issue 9, Pages 903-908

The reason of this study is to consider the removal of heavy metal lead (Pb) and cadmium (Cd) in wastewater by magnetic material using ferrite. It has been use batch experiments and operating condition are, ph(10,7and5) and time (20, 40, 60.80.100, 120) min and temperature (30) 0C after that we find the optimum condition to remove heavy metal. heavy metal in water detected by using Atomic Absorption (AA) before and after adding Cu-ferrites powder while the Cu-ferrites was characteristic by XRD and SEM ,result indicate that Cu-ferrites powder spinel structure and the particle size in range (30-40)nm ,and explain antimicrobial effectiveness of ferrites material from effect on gram positive and negative bacteria and the results appeared ability of copper ferrites to inhibition growth of both E. coli as example of gram negative bacteria and S. aureus as example of gram positive bacteria while was the E. coli survival rate of all ferrite nanoparticles concentration is more than the S. aureus survival rate , the percentage survival of bacteria was ( 35 , 20 , 12 , 5 %) in (0.625 , 1.25 , 2.5 , 5 mg/ml) concentration of microorganisms E.coli and ( 28 , 15 , 8 , 3 %) in (0.625 , 1.25 , 2.5 , 5 mg/ml) concentration for bacteria Staph. Aureus

A Restriction Enzyme from Escherichia coli Purification and General Properties

Mukaram Shikara; Nadia Tariq Barakat; Maysem Modaffer Al-Obaidy

Engineering and Technology Journal, 2009, Volume 27, Issue 5, Pages 954-961

An endonuclease restriction enzyme has been purified from E. coli about 40-fold with
DNAse and RNAse recoveries of about 3%. The purification steps included precipitation
of the enzyme with ammonium sulphate, and reclaimed it through Sephadex G-100 and
DEAE-cellulose chromatography. The purified endonuclease was able to break lambda
DNA into three bands. The enzyme has 5% of carbohydrate moiety which means it is a
glycoprotein. Lastly, the comparison with other commercial restriction endonucleases
proves that this enzyme is a restriction enzyme with enzymic activity dependent on

Role of Conjugative Plasmids in Antibiotic Resistance between Two Strains of E. coli

Raya R. G. Jabri

Engineering and Technology Journal, 2008, Volume 26, Issue 11, Pages 1429-1437

Two isolated bacterial strains (E. coli 64, E. coli MM294), were purified,
they are: gram-negative bacteria, circular, regular edged, thick somewhat
glitter, viscous, and lactose fermenter. They belong to the family of
Enterobacteriaceae E. coli 64 showed the resistant to all used antibiotics
except Rifampicin (RA), Cephotaxime (CTX), Nitrofurantion (FT),
Tabramycin (TM), and Cephalexin (RX) (Sensitive to). While E. coli
MM294 showed the sensitivity to all used antibiotics except: Rifampicin
(RA), Lincomycin (L), Clindamycin (CM), and Erythromycin (E),
(resistantt to) Conjugative experiments showed that E. coli strain 64
harbored a self transmissible plasmid carrying resistant genes for:
Tetracycline (TE), Amoxicillin (AMX), Neomycin (N), Chloramphenicol
(C), Ampicillin (AM), and Trimethoprime + Sulfamethorazole (SXT) It
seems that all these resistant genes may be carried on one self transmissible
plasmid, or they may be carried on one self transmissible plasmid (except
Neomycin, resistant gene may carry on other self transmissible plasmid)..