Print ISSN: 1681-6900

Online ISSN: 2412-0758

Keywords : DNA

DNA Fingerprints of Two Tilapia Fish Species of Euphrates River at Governorate ofAl-Muthanna Using RAPD Markers

Taha Al-Khafaji; Mustafa S.F. Ziyadi; Marwa K. Musad

Engineering and Technology Journal, 2019, Volume 37, Issue 3C, Pages 345-349
DOI: 10.30684/etj.37.3C.7

During the last decade, tilapia species (Cichlidae: Teleostei) became
members of Iraqi fish fauna. They characterized with morphological similarity among
genera and species. That makes species differentiation not easily. Molecular methods
followed to differentiate between redbelly tilapia Coptodon zillii (Gervais, 1848) and
blue tilapia Oreochromis aureus (Steindachner, 1864) of the Euphrates River sector
at Al-Samawah city. RAPD-PCR method used to create the genetic fingerprints of two
tilapia fish species. Seven decamer primers (OPA08, OPA10, OPA13, OPA17,
OPA19, OPB08 and OPC02) used to amplify DNA fragments using PCR-RAPD
technique. Forty-four bands scored after electrophoresis on 2% agarose gel along
with molecular marker fragmented to each 100 base pair. The molecular weight of
bands was calculated using PhotoCapt-MW software. The volume of bands ranged
from C. zillii 168 bp to 2227 bp while they ranged from 62 bp to 2154 bp in O.
aureus. The results achieve the RAPD fingerprints of two tilapia species in Euphrates
River at for genetic Al-Samawa city and draw the genetic tree with the same species
from Shatt A-Arab River in Basrah city. The study concluded that there is the closest
relatedness among tilapia populations from Euphrates and Shatt Al-Arab Rivers. The
results proved that RAPD markers were efficient to generate DNA fingerprints of
tilapia fish species. Furthermore, the utilizing of the RAPD markers can differentiate
the two studied species. The present study may be the first genetic study on these
tilapia fish species. Moreover, this would be the baseline studies in the future. In
addition, this study would be valuable for conservation program and documentation
of identities of tilapia fish species in Iraqi inland waters

The Study of some Plant Extracts Effect on Free Radicals Removal from DNA in Vetro

Abdul Wahid B. Al-Shaibani; Esmaeel K. Shubber; Azhar M. Haleem

Engineering and Technology Journal, 2013, Volume 31, Issue 7-, Pages 174-180
DOI: 10.30684/etj.31.7B.13

The removal efficiency of Several plant extracts of Nagilla sativa linn, Allium sativum sativum and Allium sativum ophioscordon of free radicals from pure DNA molecule at concentration 5 µgm / ml have been studied when we added 1×10-5 M of H2O2 with plant extracts mixture by measuring absorbency at 260 nanometer . Breaking index for nitrogen base pair was calculated. The plant extracts showed high ability in free radicals scavenge when used in concentrations (0.1, 1, 10, 100) µgm / ml which effect in DNA molecule. Also the DNase activity from different plant extracts was measured in DNase agar. Alcoholic mixture activity of three plants in (1،10،100) µgm/ml concentration was studied by monitoring the protection effect on pBR322 plasmid DNA from Escherichia coli which was protect t pBR322 plasmid he plasmid DNA from breaking by H2O2.