Engineering and Technology Journal

This research studies the coating layer properties from chitosan and nano –hydroxyapatite made by pulsed electrophoretic deposition (PEPD) for biomedical applications. The 316L SS alloys were coated with hydroxyapatite in different solutions in 100 mL of suspension at a 30 V continuous voltage. To characterize the deposited coatings, X-ray diffraction spectroscopy (XRD), Fourier transform infrared (FTIR), Contact Angle, scanning electron microscopy (SEM), energy dispersive x-ray analysis (EDX),Yield deposition and Optical microscopy structural (OM) was used for coating layer, in addition to being calculated Zeta potential for suspensions. The aim of this study is to show that using the Pulsed EPD method at room temperature, a simple and low-cost solution for in situ synthesis of Nano -HAp within a chitosan matrix for biomedical applications can be demonstrated.

Industrial and agricultural discharges have been shown as important sources for a wide range of contaminants like heavy metals. This study focused on the assessment of the toxic effect of heavy metals and illustrates some methods of eliminating or avoiding these contaminants. In certain methods, heavy metals (Cu, Zn, Cr, Ni, Pb, Hg, and Cd) are recovered, and range of absorbents are tested. A number of studies have reported the complex interaction of polymers with metal ions with the polymers for eliminating heavy metals (e.g. cellulose, chitosan, and chitin. Metal ions are absorbed by these polymers by the amine and hydroxyl groups. They have unique features that incorporate polyxysalt arrangement, capacity to frame films, optical underlying qualities, and chelates metal particles. Chemical bonds, pH, crystallinity, and deacetylation are controlling the mechanism and performance of the absorption. Some methods increase the efficiency, selectivity, and quality of chitosan: the first is through chemical modification employing grafting and crosslinking, and the second by physical modification, such as manufacturing gel granules and reducing crystallization. This study aims to investigate the removal of heavy metals from water by the sorption method and to study and analyze literature dealing with this topic using chitosan hydrogel.

Among the potent anticancer agents, curcumin(CU) has been found to be very efficacious against many different types of cancer cells. Chitosan-coated magnetic nanoparticles (CS MNPs) were prepared and utilized as a nano-carrier for loading of curcumin(FA-CS-CU-MNPs) througha reverse microemulsionmethod. This nanoformulation was evaluated against breast cancer cell lines in the in vitro conditions.Both shape and size properties were studied by zeta sizer, AFMand FESEM and the cell internalization ability of prepared nanoparticles was determined by fluorescence microscopy. It was found that the synthesized FA-CS-CU- MNPs were spherical in shape with an average size of 90±15 nm, low aggregation and good magnetic responsive properties. Meanwhile, the high drug loading efficiency (~73%) was remarkable. These FA-CS-CU- MNPs also demonstrated sustained release of CU at 37 ◦C in different buffer solutions. Afterwards the suitable dose and therapeutic effects of (FA-CS-CU- MNPs) for both breast cancer and normal cell lines were evaluated by MTT assay.The result showed that FA-CS-CU- MNPs retainedsignificant antitumor activities, no adverse effects was detected for normal cells.Additionally, it was observed that the FITC-labeled FA-CS-CU- MNPs could effectively enter into the cancer cells and induced cell apoptosis.