Print ISSN: 1681-6900

Online ISSN: 2412-0758

Keywords : plant extracts

The Study of some Plant Extracts Effect on Free Radicals Removal from DNA in Vetro

Abdul Wahid B. Al-Shaibani; Esmaeel K. Shubber; Azhar M. Haleem

Engineering and Technology Journal, 2013, Volume 31, Issue 7, Pages 174-180

The removal efficiency of Several plant extracts of Nagilla sativa linn, Allium sativum sativum and Allium sativum ophioscordon of free radicals from pure DNA molecule at concentration 5 µgm / ml have been studied when we added 1×10-5 M of H2O2 with plant extracts mixture by measuring absorbency at 260 nanometer . Breaking index for nitrogen base pair was calculated. The plant extracts showed high ability in free radicals scavenge when used in concentrations (0.1, 1, 10, 100) µgm / ml which effect in DNA molecule. Also the DNase activity from different plant extracts was measured in DNase agar. Alcoholic mixture activity of three plants in (1،10،100) µgm/ml concentration was studied by monitoring the protection effect on pBR322 plasmid DNA from Escherichia coli which was protect t pBR322 plasmid he plasmid DNA from breaking by H2O2.

The Study of Inhibitory Effects of Some Plant Extracts on Lymphocytes from Healthy Person

Azhar M. Haleem; Esmaeel K. Shubber; Abdul Wahid B. Al-Shaibani

Engineering and Technology Journal, 2013, Volume 31, Issue 6, Pages 139-155

The present study aimed to select plant extract or mix. of plant extracts have inhibition effect on Cancer cells from untreated CML patients the results showed the following.
Some plants extracts were used (aqueous, alcoholic, oily, alkaloid and flavonoids) from Nigella sativa seeds, Allium sativum Sativum and Allium sativum ophioscordon fruits and the inhibitory effects five different concentrations (0.1,1,10,10,1000) µg/ml was investigated on peripheral lymphocytes from healthy persons by using cytogenetic analysis BI,MI,RI,CA,SCE and CCP.
The alcoholism mix. have the greatest inhibitory effects by reducing all cytogenetic parameters, this was selected to study its effect on patient’s lymphocytes.