Print ISSN: 1681-6900

Online ISSN: 2412-0758

Keywords : Mullberry

Extraction and Characterization of A Chromosomal Stain From Black Mulberry (Morus Nigra)

Mukaram Shikara; Hiba Muneer Al-Khafagi; Wasnaa Hatif Mohammed

Engineering and Technology Journal, 2010, Volume 28, Issue 7, Pages 1432-1439

A dark-blue chromosomal stain from Black Mulberry (Morus nigra) was
extracted using organic solvents including dioxane, n-hexane, petroleum ether and
acetone. This extracted stain stained chromosomes satisfactory. In order to
ascertain purity of the extracted stain, it was compared spectrophotometery with
three commercial standards stains; they are Azur II Eosin, Giemsa’s and
Methylene blue Stains using a 200-900nm Spectrophotometric scan at three
different concentrations (25, 50 and 100mg/ml).
It was found that the second peaks produced by Azur II eosin and Giemsa
stain (as well as the single peak produces by Methylene blue) occupied the same
area occupied by the second peak of the extracted stain, which agree to some
degree of similarity. The extracted method proved to be an appropriate analytical
technique with hope that further trials can be carrying out to simplify the
extracting of the stain in future researches and make it less expensive.