Print ISSN: 1681-6900

Online ISSN: 2412-0758

Keywords : Antibiotics

Study of the Antibacterial Activity by using Titanium Dioxide Nanoparticles

Kareem Hussian AL Shibaib

Engineering and Technology Journal, 2016, Volume 34, Issue 6, Pages 198-203

The study was the effectiveness of the microbial activity by using titanium dioxide nanoparticles which are prepared by using laser ablation of titanium pellet immersed in de-ionzed water. Palsed Nd: YAG laser with 1064nm wavelength and laser energy 250 mJ was used to irradiate the target for ablation time 15 min, 200 pulses. Where studied synthetic characteristics of the sample prepared using the X-ray diffraction pattern of nanoparticles proved the presence Alatanas planes. A scanning electron microscope measurement appearance Tio2 nanoparticle of structures with cube shapes , while the results of Zeta potential was the values being equal to (34) are located within the range of suspension potential Stability. The results of antimicrobial activity of Tio2 nanoparticle against bacterial stratin S.aureus, C .albicans, and E.coli with antibiotic at concentration .1000 mg/ml (AMC, DO10 and CN10) using well diffusion assay showed. The best concentration of 1000 mg /ml of antibiotics give us with dioxide nanoparticles to inhibition bacterial growth .The results showed that the nanoparticles with antibiotics inhibition the growth of S.aureus then C.albicans which is more sensitive than E.coli .Our results showed that dioxide nanoparticles have synergistic effect with (CN10& DO10) .And there is no any synergistic effect with (AMC).

Effect of Antibiotics, Anesthetics, Sugars and Saltson The Oxidation Activity of Human Ceruloplasmin in vitro

Ali J.Al - Sa; ady; Dalal S. Bedan

Engineering and Technology Journal, 2016, Volume 34, Issue Issue 5, Pages 730-737

The optimum conditions for Ceruloplasmin (CP) activity have been measured in serum samples of a healthy individual without any detectable diseases. CP activity was evaluated by measurement of its p-phenylenediamine oxidase activity. This study showed the optimum pHforCP activity of a healthy human serum was 6.0 and the optimal temperature for CP activity was 45°C using in vitro conditions. The maximum value of oxidation activity was in incubation period70 min using optimal substrate concentration 60 mmol/l.
Several types of antibiotics were experimented in order to detect their effect on ceruloplasmin oxidation activity; these are consisted of Cephalexin, Amoxicillin, Erythromycin, Ampicillin, Ampicillin & Cloxacillin, Streptomycin Sulfate, Chloramphenicol, Cefrtriaxone, Benzathine, Pencilline and Aspegic. Results showed that Ampicillin and Streptomycin Sulfate had maximum influence on ceruloplasmin activity. Different types of sugars, salts and anesthetics were used to evaluate their effect on p-phenylenediamine oxidation by serum ceruloplasmin. Results appeared that starch, FeSo4 and lidocaine 4% were activator for oxidation process. This study aimed to measure the optimal conditions of ceruloplasmin activity and to evaluate effect of different antibiotics and anesthetics onproteins from the serum of a healthy individual using in vitro conditions.