Silver nitrate and commercial sigma Nisin were used to synthesize Nisin-Silver nanoparticles; different concentrations of Nisin at different pH conditions were used to optimize the characterization.10 μg/ml at pH 6.0 had the greatest observation and the deferent conditions characterization of Nisin-Silver nanoparticles agreed with that different methods were used to prepare the Nisin-Silver nanoparticles and the results showed that the best method to prepare the nanoparticles (Nisin-Silver) was sunlight method.