In this work, NiO thin films have synthesized by pulsed laser deposition on glass substrates with different substrate temperature (100, 200, 300)°C, using Q-switching Nd:YAG laser. Structure and optical properties have carried out by using FTIR, AFM and UV- Vis spectroscopy. FTIR spectraconformed of NiO bonding and AFM images show the increase in grain size with temperature. The optical transmission results show that the transparency of the NiO films is greater than 85% in the visible region which increases with the increasing substrate temperature, While the energy band gap was decreased with increasing substrate temperature.