In this work, synthesis of CdS nanoparticles with Aloe vera (L.) extract and its activity on bacterial pathogens was investigated. CdS nanoparticles were synthesized by using chemical methods. Physical properties like: X- ray diffraction (XRD), Atomic force microscopy (AFM) and UV-VIS transmission spectroscopy were employed to characterize the crystalline structure, size and transmission spectra of nanoparticles. X-ray diffraction analysis confirms the formation of cubical and hexagonal structures of CdS nanoparticle. The AFM show the formation of aggregate of nanoparticle with particle size ranging 7 – 20 (nm). The antibacterial activity of Aloe vera-conjugated CdS nanoparticles showed effective inhibitory activity against the pathogens.