In this research we study the effect of different extracts of Teucrium polium (cold water, hot water and alcohol extraction), effect of silver nanoparticles and the combination effect of Teucrium polium extracts with silver nanoparticles against different pathogenic microorganisms Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus volgaris, Staphylococcus aureus, Streptococcus and Candida albicans. The results showed that the effect of cold water extract on microbial activity only inhibited the growth of P. aeruginosa. In case of hot water extract there is no effect on any type of microorganisms used in this study unlike the ethanolic extract it is affect on the growth of all microorganisms used except P. volgaris and E. coli. In the using of silver nanoparticles it inhibits the growth of all the microorganisms used in this research but in different ratios. In case of combination the effect will increase with assistant of silver nanoparticles except in P. aeruginosa and K. pneumonia the effect of silver nanoparticles alone is higher