This project aimed to increase the production of some secondary metabolites using physical and chemical elicitors in tissue cultures of Hypercom triquetrifolium L.. The quality and quantity of photochemical were estimated using methanolic extracts of dried leaves and callus were analyses using HPLC. Callus was initiated on leaf discs cultured on Murashig and Skoog (MS) medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) and Benzyl adenosine (BA) at concentrations of 0,0.1, 0.5, 2.0 or 5.0 mg/l for H. triquetrifolium callus. Results showed that the combination of 2,4-D at 0.1 mg/l with BA at 0.5 mg/l was the most effective for callus induction percentage reached 90%. The highest mean fresh weight reached 64.33 mg for H. triquetrfolium. The same combination was used for callus maintenance for plants. Results also showed an increase in the concentration of secondary metabolites in methanol extracts induced on leaves. Callus cultures induced on leaf discs were treated with some physical stimuli such as light, ultraviolet, the different exposure to photoperiod (dark for 24 hrs, 12 hrs light, 16 hrs light or 24 hrs light), the uv exposure time was 10 or 20 minutes. Result showed that there are significant differences between the various treatmeant, The best light exposure time caused an increase in the production of secondary metabolism was 12 hrs light for calli in both plants. Both exposure times (10 or 20 mint) induced the yield of secondary metabolites in callus cultures of H. triquetrifolium equally.