In this study, polyvinyl alcohol composite polymer membrane was successfully synthesized pure and at different TiO2 concentrations (1%, 3%). The diameter of the PVA–TiO2fibers ranged from 100 to 200 nm. X-ray diffraction analysis indicated that the main crystal structure was amorphous and there are no any sign of Anatase or Rutile or Brookite when increased TiO2 concentrations to3% .absorption properties of polymeric membranes doped absorb of increases compare with of the undoped membrane. PVA pure nano-fibers membrane less zone of inhibition than PVA : 0.1 TiO2nano-fibers membrane and PVA : 0.3 TiO2nano-fibers membrane against gram-negative(Pseudomonas and Salmonella) and gram-positive (Staphylococcus aureus (S. aureus)).