Antibacterial fabrics were papered by using cotton fibers, CuSO4: 5H2O, ZnSO4 and TiO2. These cotton fibers were coated by nano thin film consists of titanium oxide, copper oxide and zinc oxide. The study of structural properties by XRD had indicated that the coated fabric thin film was polycrystalline. The topography of the thin films surface was identified by using a scanning electronic microscope (SEM) and optical microscope (OM). Optical properties were measured using optical spectrometer, the optical properties improve lead to increase in the effectiveness of anti-bacterial fabric during add titanium dioxide and zinc oxide to coated fabric by copper oxide, as well as improve the biological activity of fabric the thin film using the substance added (Methanol Sulphate) to a chemical solution during the initial preparation process of the coating fabric by oxide copper thin film. Biological characteristics have been studied for fabric against bacteria types Escherichia coli, Staphylococcus aureus and Bacillus cereus.