Electrospinning has recently emerged as a leading technique for generating biomimetic scaffolds made of synthetic and natural polymers for tissue engineering applications. PVA was dissolved in distilled water at concentration (10% wt). PVA 10% DW solution was prepared as biopolymeric materials for fabricating tissue engineered scaffolds by electrospinning, varying ambient temperature (25, 30, 35, 40, 45 and 50) ͦ C andinvestigated the ambient temperature effect on tissue nanofibers pore size. Scanning electron microscopy was utilized to profile the topography of individual electrospun fibers. Statistical measurements for each SEM images lead to measure the mean poresize of tissue obtained. Our results indicate that the average poresize of PVA fibertisues could be scaled down to mean values (132-300)nm smooth nanofibers without any beads, poresize decreased as ambient temperature increase to certain temperature at (40 °C) and retrain increasing at (45 and 50) °C temperature.