In this study, hybridmaterial like ZnO coated multi-walled carbon nanotubes (ZnO/MWCNTs) was formation by a multistep chemical procedure and evaluated for their application potentials as an antimicrobial agent for slowing down bacterial growth as bacteriostatic and inactivating pathogenic bacteria as bactericidal. The prepared carbon nanotubes and ZnO nanoparticles were investigated by using X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). The antimicrobial properties of hybrid material (ZnO/MWCNTs) were tested against Salmonella typhi (S.typhi), Pseudomonas aeruginosa (P. aeruginosa) which are Gram-negative and Staphylococcus aureus (S. aureus) which is Gram-positive by using standard agar dilution (plate count) method.