In this research, Sol-gel technique prepared Ag–TiO2 nano-composite thin films, which were deposited onto a glass substrate by the spin coating process. The microstructures and chemical ingredients of the obtained thin films were characterized by UV-visible spectroscopy (UV), Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), and Fourier Transform Infrared Spectroscopy (FT-IR). It was discovered that the silver nano-particles completely joined to the TiO2 matrix, where those nanoparticles distributed uniformly. In this way, the molar percent of the silver nitrate watery solution dominated the morphology of the thin film. Ag-TiO2 Nano composite is very useful for expanding antibacterial of nanomaterials purpose.