The optimum conditions for Ceruloplasmin (CP) activity have been measured in serum samples of a healthy individual without any detectable diseases. CP activity was evaluated by measurement of its p-phenylenediamine oxidase activity. This study showed the optimum pHforCP activity of a healthy human serum was 6.0 and the optimal temperature for CP activity was 45°C using in vitro conditions. The maximum value of oxidation activity was in incubation period70 min using optimal substrate concentration 60 mmol/l.
Several types of antibiotics were experimented in order to detect their effect on ceruloplasmin oxidation activity; these are consisted of Cephalexin, Amoxicillin, Erythromycin, Ampicillin, Ampicillin & Cloxacillin, Streptomycin Sulfate, Chloramphenicol, Cefrtriaxone, Benzathine, Pencilline and Aspegic. Results showed that Ampicillin and Streptomycin Sulfate had maximum influence on ceruloplasmin activity. Different types of sugars, salts and anesthetics were used to evaluate their effect on p-phenylenediamine oxidation by serum ceruloplasmin. Results appeared that starch, FeSo4 and lidocaine 4% were activator for oxidation process. This study aimed to measure the optimal conditions of ceruloplasmin activity and to evaluate effect of different antibiotics and anesthetics onproteins from the serum of a healthy individual using in vitro conditions.