In this study Zinc oxide Nano particles was synthesized by employing sol-gel technique to explore novel antifungal agents to beat the developed of fungi resistance to many conventional fungicides .Prepared of Zinc oxide Nano particles were investigated via using Scanning Electron Microscopy (SEM, the VEGA Easy Probe), X ray powder diffraction (XRD) , FTIR spectrum, The antifungal properties of ZnO nanoparticles were tested against Fussarium spp., Alternaria spp. , Penicillium spp. , and Aspergillus spp. by using agar diffusion method. The percentage of growth was lowest comparison with control for all types of fungi when used zinc oxide Nano in different concentration.