Document Type : Research Paper


1 College of Science for Women, University of Baghdad -Iraq

2 College of Education for Pure Science, University of Tikrit - Iraq

3 College of Science, University of Salahddin - Iraq


The main objective of the present investigation was to ascertain of different phytochemicals in two ferns: Asplenium scolopendrium L. from Malakan- Erbil district and A. trichomanes L. from Tweela-Sulaymaniyah district during Spring 2016. Antioxidant activity and total flavonoids contents were determined in methanol extracts. In qualitative analysis, the active compounds such as alkaloids, tanins, saponins and flavonoids were screened. In quantitative analysis, total flavonoids were quantified 234.7 µg /ml with IC50 of 113 mg/ml in A. scolopendrium but in A. trichomanes total flavonoids 1061µg/ml. with IC50 of 2.271 mg/ml comparison with Ascorbic acid IC50 of 0.0186 mg/ml as positive control.


[1] A. Sasaki, “Aspleniaceae. In: National Museum of
Nature and Science, Japan (ed.), Illustrated Flora of Ferns &
Fern-allies of South Pacific Islands,” Tokai University
Press, Hatano, pp. 133-146, 2008 (in Japanese).
[2] D.H. Gracelin, A.J. De Britto and P.R. Benjamin,
Qualitative and quantitative analysis of phytochemicals in
five Pteris species,” International Journal of pharmacy and
pharmaceutical sciences, Vol. 5, No. 1, pp. 105-107. 2013.
[3] M.E. Alonso-Amelot, A. Oliveros, P.M Calcagno, and
E.B. Arellano, “adaptation mechanisms and xenobiotic
chemistry,” Pure and Applied chemistry, Vol. 73, pp.549-
553, 2001.
[4] C.D. Preston, & D.A. Pearman, “New atlas of the
British and Irish flora,” Oxford Univ. Press.2002.
[5] A.M. Ismail, B.K. Maulood, and T.O. Al- Khesraji,
“An ecological and morphological study on Asplenium
scolopendrium L. in Erbil city- Kurdistan of Iraq,” Journal
of Garmian Univ, Vol. 163, pp. 555-562, 2017.
[6] R. Ho, T. Teai, J.P. Bianchini, R. Lafont, and P.
Raharivelomanana, “Ferns: From Traditional Uses to
Pharmaceutical Development, Chemical Identification of
Active Principles, working with ferns,” Springer, New
York, NY. Pp. 321–346, 2011 .
[7] S. Mir, K Mishra, A., Reshi, Z. and M. Sharma,
“Preleminary phytochemical screening of some
pteridophyts from district Shopian (j & k),” International J
of pharmacy and pharmaceutical sciences, Vol. 5.2013.
[8] J. Andrade, R. Dresch, C. doss Passos, et al.,
“Chemical analysis and antioxidant, antichemotactic and
monoamininhyibitio effects of some pteridophyts from
Brazil,” phrmacognocy Magazin, Vol. 10, No. 37, pp. 100,
[9] J.B. Harborne, “Phytochemical methods: A guide to
modern techniques of plant analysis,” New York, Chapman
and Hall, 3, pp. 1-150, 1998.
[10] B. Suarez, N. Palacios, N. Fraga, and R. Rodrigues,
“Liquid chromatographic method for quantifying
polyphenols in ciders by injection,” Journal of
Chromatography A, 1066, pp.105-110, 2005.
[11] Z.Chen, R.Bertin, and G. Froldi, “EC50 Estimation of
antioxidant activity in DPPH assay using several statistical
programs,” Food Chem, Vol. 138, No. 1, pp. 414-420, 2013.
[12] M.E. Alonso-Amelot, A. Oliveros, and M.B.
Calcagno, “ Phenolics and condensed tannins in relation to
altitude in neotropical Pteridium spp, A field study in
Venezuelan Andes,” Biochemical Systematic and Ecology,
Vol. 32, pp. 969-981, 2004.
[13] J.M. Moteiro, U.P. Albuquerque, Lins Neto, EMF,E.
LAraujo, M.M. Albuquerque, and E.L.C. Amorim, “The
effects of seasonal climate changes in the Caatinga on
tannins level,” Rev.Bras.Farmacogn, Vol. 16, pp. 338-344,
[14] L.L. Borges, S.F. Alves, B.L. Sampaio, E.C.
Conceicao, M.T.F. Bara, and J.R. Paula, “ Environmental
factors affecting the concentration of phenolic compounds
in Myrcia tomentosa leaves, ” Brazilian Journal of
Pharmacognosy, Vol. 23, No. 2, pp. 230-238, 2013
[15] L. Gobbo-Neto,.and N.P. Lopez, “ Plants medicinal
factors de influencia no conteudo de metabolitos
secondarios,” Quim Nova, Vol. 30, pp. 374-381, 2007
[16] J. Mierziak, K. Kostyn, and A. Kulma, “Flavonoids
as important molecules of plant interaction with
environment,” Molecules, Vol. 19, pp. 16240-16265, 2014.
[17] A. Ghashemzadeh, and N. Ghashemzadeh,
“Flavonoids and phenolic acid role and biological activity in
plants and human,” J .med. pl. Res., Vol. 5, No. 31, pp.
6697-6703, 2011.
[18] S.S. Ali, N. Kasoja, A. Luthru et al., “Indian
medicinal plants as sources of antioxidants,” Food Res. Int.,
Vol. 41, pp. 1-25, 2008.
[19] M.G. Santos,A. Kelecom, S. Riberodeparia, et al.,
“Phytochemical studies in pteridophyts growing in Brazil:
A review,” The American J of Pl. Sci. and Biotec., Vol. 4
(special Issue 1), pp. 113-125, 2010.
[20] C. Yung-Husan, C. Fang-Rong, L. Yih-Jer. et al.,
“Identification of phenolic antioxidants from Sword Bark
fern (Pteris enciforms Burm.),” Food Chem., Vol. 105,
pp.48-56, 2007.
[21] T. Chai, C. Elamparothi, A. Young, Y. Quah, and F.
Wong, “Antibacterial, anti-glucosidase, and antioxidant
activity of selected highland ferns of Malaysian,” Botanical
Studies, Vol. 54, pp. 55-61, 2013.
[22] A.J. De Britto, D.H. Gracelin, & P.B. Kumar, “
Phytochemical studies on five of ferns collected Southern
Western Ghats, Tamilnuda,” Asian Pacific Journal Tropical
Biomedicine, Vol. 2, No. 2, pp.5536-5538, 2012.