Document Type : Research Paper


1 Environmental Engineering Department, University of Baghdad - Iraq

2 Chemical Engineering Department, Al-Nahrain University - Iraq


The aim of the present study is to isolate and recognize calcium
carbonate-producing bacteria and to check these bacterial strains for use in
cement mortar to improve its properties such as strength and water absorption.
Bacteria were isolated from livestock soils were examined for urease producing
activity, the possibility to precipitate calcite and its activity to improve the
compressive strength. Based on the results, four isolates were selected and
identified. They were characterized as B. atrophaeus, B. subtilis, B. aryabhattai
and B. amyloliquefaciens. Experimental work was performed to evaluate the
effect of bacterial concentration in term of optical density (OD) on the
compressive strength. Bacterial cement mortar samples revealed improvement
in compressive strength and water sorptivity. The efficiency of bacterial strains
towards crack remediation was also investigated. Considerable increase in
compressive strength and complete cracks remediation was detected in cement
mortar samples cured with all bacteria isolate (using OD=1). This specifies the
suitability of these bacteria for use in cement mortar. The precipitate of calcium
carbonate inside the cracks of cement mortar by bacterial isolate was analyzed
under a scanning electron microscope (SEM)


[1] P. López-García, J. Kazmierczak, K. Benzerara, S.
Kempe, F. Guyot, and D. Moreira, “Bacterial diversity
and carbonate precipitation in the giant microbialites
from the highly alkaline Lake Van, Turkey,"
Extremophiles, Vol. 9, No. 4, pp. 263-274, 2005.
[2] J. Zhang et al., “Screening of bacteria for selfhealing of concrete cracks and optimization of the
microbial calcium precipitation process," Applied
microbiology and biotechnology, Vol. 100, No. 15, pp.
6661-6670, 2016.
[3] Y. Shen, R. Buick, and D. E. Canfield, “Isotopic
evidence for microbial sulphate reduction in the early
Archaean era," Nature, Vol. 410, No. 6824, p. 77, 2001.
[4] D. S. McKay et al., “Search for past life on Mars:
Possible relic biogenic activity in Martian meteorite
ALH84001," Science, Vol. 273, No. 5277, pp. 924-930,
[5] B. Lian, Q. Hu, J. Chen, J. Ji, and H.H. Teng,
“Carbonate biomineralization induced by soil
bacterium Bacillus megaterium," Geochimica et
Cosmochimica Acta, Vol. 70, No. 22, pp. 5522-5535,
[6] I. Hammad, F. Talkhan, and A. Zoheir, “Urease
activity and induction of calcium carbonate
precipitation by Sporosarcina pasteurii NCIMB 8841,"
Journal of Applied Sciences Research, Vol. 9, No. 3, pp.
1525-1533, 2013.
[7] R.K. Verma, L. Chaurasia, V. Bisht, and M.
Thakur, “Bio-mineralization and bacterial carbonate
precipitation in mortar and concrete," Bioscience And
Bioengineering, Roorkee, India, Vol. 1, No. 1, pp. 5-11,
[8] F. Hammes and W. Verstraete, “Key roles of pH
and calcium metabolism in microbial carbonate
precipitation," Reviews in environmental science and
biotechnology, Vol. 1, No. 1, pp. 3-7, 2002.
[9] A. Gurbuz, Y.D. Sari, Z.N. Yuksekdag, and B.
Cinar, “Cementation in a matrix of loose sandy soil
using biological treatment method," African Journal of
Biotechnology, Vol. 10, No. 38, pp. 7432-7440, 2011.
[10] S. Amidi and J. Wang, “Surface treatment of
concrete bricks using calcium carbonate precipitation,"
Construction and Building Materials, Vol. 80, pp. 273-
278, 2015.
[11] N.K. Dhami, M.S. Reddy, and A. Mukherjee,
“Improvement in strength properties of ash bricks by
bacterial calcite," Ecological Engineering, Vol. 39, pp.
31-35, 2012.
[12] S.K. Ramachandran, V. Ramakrishnan, and S.S.
Bang, “Remediation of concrete using microorganisms," ACI Materials Journal-American Concrete
Institute, Vol. 98, No. 1, pp. 3-9, 2001.
[13] K. Van Tittelboom, N. De Belie, W. De Muynck,
and W. Verstraete, “Use of bacteria to repair cracks in
concrete," Cement and Concrete Research, Vol. 40, No.
1, pp. 157-166, 2010.
[14] V. Ramakrishnan, K. Deo, E. Duke, and S. Bang,
“SEM investigation of microbial calcite precipitation in
cement," in Proceedings of the International
Conference on Cement Microscopy, vol. 21, 1999.
[15] P. Ghosh, S. Mandal, B. Chattopadhyay, and S.
Pal, “Use of microorganism to improve the strength of
cement mortar," Cement and Concrete Research, Vol.
35, No. 10, pp. 1980-1983, 2005.
[16] V. Achal, A. Mukherjee, P. Basu, and M. S. Reddy,
“Lactose mother liquor as an alternative nutrient source
for microbial concrete production by Sporosarcina
pasteurii," Journal of industrial microbiology &
biotechnology, Vol. 36, No. 3, pp. 433-438, 2009.
[17] V. Achal, A. Mukherjee, and M. S. Reddy,
“Microbial concrete: way to enhance the durability of
building structures," Journal of materials in civil
engineering, Vol. 23, No. 6, pp. 730-734, 2010.
[18] S. Ghosh, B. Chattopadhyay, and S. Mandal, “Use
of hot spring bacteria for remediation of cracks and
increment of durability of structures," Indian concrete
journal, Vol. 82, No. 9, pp. 11-16, 2008.
[19] S. Krishnapriya and D. V. Babu, “Isolation and
identification of bacteria to improve the strength of
concrete," Microbiological research, Vol. 174, pp. 48-
55, 2015.
[20] V. Ramakrishnan, R. K. Panchalan, S. S. Bang, and
R. City, “Improvement of concrete durability by
bacterial mineral precipitation," in Proceedings of 11th
International Conference on Fracture, 2005, pp. 20-25.
[21] W. De Muynck, D. Debrouwer, N. De Belie, and
W. Verstraete, “Bacterial carbonate precipitation
improves the durability of cementitious materials,"
Cement and concrete Research, Vol. 38, No. 7, pp.
1005-1014, 2008.
[22] G. Halket, A. Dinsdale, and N. Logan, “Evaluation
of the VITEK2 BCL card for identification of Bacillus
species and other aerobic endosporeformers," Letters in
applied microbiology, Vol. 50, No. 1, pp. 120-126,
[23] V.S. Whiffin, L.A. van Paassen, and M.P. Harkes,
“Microbial carbonate precipitation as a soil
improvement technique," Geomicrobiology Journal,
Vol. 24, No. 5, pp. 417-423, 2007.
[24] T. Gonen and S. Yazicioglu, “The influence of
compaction pores on sorptivity and carbonation of
concrete," Construction and building materials, Vol.
21, No. 5, pp. 1040-1045, 2007.
[25] C. ASTM, “1585-04. Standard test method for
measurement of rate of absorption of water by
hydraulic-cement concretes," ASTM International,
[26] S. Abo-El-Enein, A. Ali, F.N. Talkhan, and H.
Abdel-Gawwad, “Utilization of microbial induced
calcite precipitation for sand consolidation and mortar
crack remediation," HBRC Journal, Vol. 8, No. 3, pp.
185-192, 2012.
[27] İ. Türkmen, “Influence of different curing
conditions on the physical and mechanical properties of
concretes with admixtures of silica fume and blast
furnace slag," Materials Letters, Vol. 57, No. 29, pp.
4560-4569, 2003.
[28] A.S.F. Testing and M.C.C. Cement, "Standard Test
Method for Compressive Strength of Hydraulic Cement
Mortars (using 2-in. Or [50-mm] Cube Specimens).
ASTM International," 2013.