Document Type : Research Paper
Authors
1 College of Agriculture, University of Muthanna Dept. Animal Production Al-Muthanna -Iraq
2 Marine Science Centre, University of Basrah Dept. Marine Vertebrate Basra -Iraq
3 college of Agriculture, University of Muthanna Dept. Animal Production Al-Muthanna -Iraq
Abstract
During the last decade, tilapia species (Cichlidae: Teleostei) became
members of Iraqi fish fauna. They characterized with morphological similarity among
genera and species. That makes species differentiation not easily. Molecular methods
followed to differentiate between redbelly tilapia Coptodon zillii (Gervais, 1848) and
blue tilapia Oreochromis aureus (Steindachner, 1864) of the Euphrates River sector
at Al-Samawah city. RAPD-PCR method used to create the genetic fingerprints of two
tilapia fish species. Seven decamer primers (OPA08, OPA10, OPA13, OPA17,
OPA19, OPB08 and OPC02) used to amplify DNA fragments using PCR-RAPD
technique. Forty-four bands scored after electrophoresis on 2% agarose gel along
with molecular marker fragmented to each 100 base pair. The molecular weight of
bands was calculated using PhotoCapt-MW software. The volume of bands ranged
from C. zillii 168 bp to 2227 bp while they ranged from 62 bp to 2154 bp in O.
aureus. The results achieve the RAPD fingerprints of two tilapia species in Euphrates
River at for genetic Al-Samawa city and draw the genetic tree with the same species
from Shatt A-Arab River in Basrah city. The study concluded that there is the closest
relatedness among tilapia populations from Euphrates and Shatt Al-Arab Rivers. The
results proved that RAPD markers were efficient to generate DNA fingerprints of
tilapia fish species. Furthermore, the utilizing of the RAPD markers can differentiate
the two studied species. The present study may be the first genetic study on these
tilapia fish species. Moreover, this would be the baseline studies in the future. In
addition, this study would be valuable for conservation program and documentation
of identities of tilapia fish species in Iraqi inland waters
Keywords
Main Subjects
“Structural Diversity of Fish Assemblage in the Southern
Sector of Main Outfall Drains northwest of Basrah, Iraq,”
Basrah J. Agric. Sci., Vol. 31, No. 1, pp. 1-11, 2018.
[2] Kh. E. Saleh, “New record of Tilapia zilli in Iraqi
natural waters in Euphrates River” 1
st Scientific
Conference of Agriculture, College of AgricultureUniversity of Basra. (Arabic abstract), 2007.
[3] F.M. Mutlak, and A.J. Al-Faisal, “New record of two
exotic cichlid fish Oreochromis aureus (Steindachner,
1864) and Tilapia zilli (Gervais, 1848) from south of the
main outfall drain in Basrah city,” Mesop. J. Mar. Sci.,
Vol. 24, No. 2, pp. 160 – 170, 2009.
[4] A.J. Al-Faisal, and F.M. Mutlak, “First record of the
Nile tilapia Oreochromis niloticus (Linnaeus, 1758), from
the Shatt Al-Arab River, Southern Iraq,” International
Journal of Marine Science, Vol. 5, No. 38, pp. 1-3, 2014.
[5] J. De Maeseneer, “The culture of Tilapia species in
tropical and subtropical conditions,” Tropiculture, Vol. 2,
No. 1, pp. 19-25, 1984.
[6] FAO. “Aquaculture Production, Year Book of Fishery
Statistics,” - Vol. 96/2. Food and Agriculture Organization
of the United Nations, Rome, Italy, 125 p., 2005.
http://www.fao.org/fishery/publications/yearbo
oks/en.
[7] C.W. Martin, M.W. Valentine, J.F. Valentine,
“Competitive Interactions between Invasive Nile Tilapia
and Native Fish: The Potential for Altered Trophic
Exchange and Modification of Food Webs,” PLoS ONE,
Vol. 5, No. 12, pp. e14395, 2010.
[8] P. Sodsuk, and B.J. McAndrew, “Molecular
systematics of three tilapia genera Tilapia, Sarotherodon
and Oreochromis using allozyme data,” J. of Fish Biol.,
Vol. 39, pp., 301-308, 1991.
[9] S. Seyoum, “Allozyme variation in subspecies of
Oreochromis niloticus (Pisces: Cichlidae),” Isozyme Bull.,
Vol. 23, pp., 97, 1990.
[10] J.F. Agnese, B., Adépo-Gourène, E.K. Abban, and Y.
Fermon, “Genetic differentiation among natural
populations of the Nile tilapia Oreochromis niloticus
(Teleostei, Cichlidae),” Heredity, Vol. 79, pp. 88–96, 1997.
[11] M. Lynch, and B.G. Milligan, “Analysis of population
genetic structure with RAPD markers,” Molecular
Ecology, Vol. 3, pp. 91-99, 1994.
[12] J. Welsh, and M. McClelland, “Fingerprinting
genomes using PCR with arbitrary primers,” Nucleic Acids
Research, Vol. 18, No. 24, pp. 723-7218, 1990.
[13] J.G.K. Williams, A.R. Kubelik, K.J. Livak, J.A.
Rafalski, and S.V. Tingey, “DNA polymorphisms
amplified by arbitrary primers are useful as genetic
markers,” Nucleic Acids Research, Vol. 18, No. 22, pp.
6531-6535, 1990.
[14] H. Hadrys, M. Balick, and B. Schierwater,
“Applications of random amplified polymorphic DNA
(RAPD) in molecular ecology,” Mol. Ecol., Vol. 1, pp. 55-
63, 1992.
[15] F. Bardakci, and D.O.F. Skibinski, “Application of
RAPD technique in tilapia fish: species and subspecies
identification,” Heredity, Vol. 73, pp. 117-123, 1994.
[16] K.R., Dinesh, T.M. Lim, W.K. Chan, and V.P.E.
Phang, “Genetic variation inferred from RAPD
fingerprinting in three species of tilapia,” Aquaculture
International, Vol. 4, 19-30, 1996.
[17] M.M.M. Ahmed, B.A. Ali, and S.Y. EI-Zaeem,
“Application of RAPD markers in fish: Part I – some
genera (Tilapia, Sarotherodon, and Oreochromis) and
species (Oreochromis aureus and Oreochromis niloticus)
of Tilapia,” Int. J. Biotechnology, Vol. 6, No. 1, pp.86–93,
2004.
[18] C. Callejas, and M.D. Ochando, “Identification of
Spanish barbell species using the RAPD technique,” J.
Fish Biol. Vol. 53, pp. 208-215, 1998.
[19] C. Callejas, and M.D. Ochando, “Molecular
identification (RAPD) of the eight species of the genus
Barbus (Cyprinidae) in the Iberian Peninsula,” J. Fish
Biol., Vol. 59, pp. 1589-1599, 2001.
[20] C. Callejas, and M.D. Ochando, “Phylogenetic
relationships among Spanish Barbus species (Pisces,
Cyprinidae) shown by RAPD markers,” Heredity, Vol.
89:36-43, 2002.
[21] Z. Mamuris, C. Stamatis, and C. Triantaphyllidis,
“Intraspecific genetic variation of striped red mullet
(Mullus surmuletus L.) in the Mediterranean Sea assessed
by allozyme and random amplified polymorphic DNA
(RAPD) analysis,” Heredity, Vol. 83, pp. 30-38, 1999.
[22] J-M. Yoon and J-W., Kim “Randomly amplified
polymorphic DNA-polymerase chain reaction analysis of
two different populations of cultured Korean catfish
Silurus asotus.” J. Biosciences, Vol. 26, No. 5, pp. 641-
647., 2001.
[23] D. Jini, “Genotypic Analysis of Etroplus Maculatus in
Western Ghat Rivers of India using Random Amplification
of Polymorphic DNA (RAPD) analysis,” J. of Genetics
and Genetic Engineering Vol. 1, No. 1, PP. 1-8, 2017.
[24] M.S. Faddagh, N.A. Husain and A.I. Al-Badran,
“DNA fingerprinting of eight cyprinid fish species of Iraqi
inland waters using RAPD-PCR,” Advances in Life
Sciences, Vol. 2, No. 2, pp. 9-16, 2012.
[25] M.S. Faddagh, “Usage of new decamer primer MB1-
9 to discriminate six Barbus fish species (Cyprinidae:
Teleostei) in Iraqi freshwaters,” Basrah J. Research
Sciences, Vol. 40, No. 3, Part A, pp. 148-155, 2014.
[26] M.S. Faddagh, “Genetic Diversity of Kattan
Luciobarbus xanthopterus Heckel, 1843 (Pisces:
Cyprinidae) in Four Mesopotamian Inland Waters, Iraq,”
Basrah J. Agric. Sci., Vol. 29, No. 1, pp, 25-33, 2016.
[27] A.J. Al-Faisal, A.R. M. Mohamed, T. A. Jaayid,
“New record of the carangid fish from the Iraqi marine
waters, northwest Arabian Gulf” Mesop. Environ. J.,
special issue A, pp. 106-114, 2016.
[28] FishBase, “Fishbase.org/ Identification Keys, 2019
[online]. https://www.fishbase.se/keys/allkeys.php.
[29] UPGMA, “Unweighted Pair Group Method with
Arithmetic Mean [online].
http://genomes.urv.cat/UPGMA/, 2016.
[30] MS. Faddagh, T.Y. Al-Khafaji, and M.K. Musaad,
“DNA fingerprints of Tilapia species in Shatt Al-Arab
River using RAPD markers,” Unpublished.
[31] J. Briolay, N. Galtier, R.M. Brito and Y. Bouvet
“Molecular phylogeny of Cyprinidae inferred from
cytochrome b DNA sequences,” Mol. Phylogenet. Evol.,
Vol. 9, No. 1, pp. 100-108, 1998.
[32] A. Machordom, I. Doadrio, and P. Berrebi,
“Phylogeny and evolution of the genus Barbus in the
Iberian Peninsula as revealed by allozyme electrophoresis”
J. Fish Biol., Vol. 47, No. 2, pp. 211-236, 1995.
[33] O.H.M. Shair, R.M. Al- Ssum, A.H. Bahkali,
“Genetic variation investigation of tilapia grown under
Saudi Arabian controlled environment,” Am. J. Biochem.
Mol. Biol, Vol. 1, pp. 89-94, 2011.
[34] B. Neekhra, A.A. Mansoori, S. Verma, R.K. Koiri
and S.K. Jain, “RAPD-PCR Based Biomarker Study in
Fish Species (Family: Cyprinidae) of Madhya Pradesh,
India,” Austin J. Mol & Cell Biol. Vol. 1, No. 1, pp. 1003,
2014.
)
