Authors
1 Applied Sciences Department, University of Technology, Baghdad, Iraq.
2 Applied Sciences Department, University of Technology, Baghdad, Iraq
Abstract
The activity of urease purified from Proteus mirabilis bacteria was
estimated using gamma iron oxide nanoparticles (γ-Fe2O3) which were obtained as
standard in size (20- 40)nm, purity 99%. The enzyme activity was estimated by
incubating the pure enzyme with various concentrations of nanoparticles ranged
between (1- 6) μg/ml. The results indicates a decrease in enzymatic activity with
increasing of nanoparticles concentration. After that, the concentration of blood
urea was measured using urease obtained from a standard kit, urease purified from
Proteus mirabilis and the urease- gamma iron nanoparticles solution. The
comparison was then made among the results of urea concentrations by statistical
analysis using T-test. The results showed that there is no significant difference
between the results obtained from urease standard kit and urease purified from
the bacteria, this is due to the efficiency of urease purified from the bacteria. On the
other hand, the results showed that there is a significant difference (P≤ 0.01) in
urea concentrations obtained from urease- gamma iron oxide nanoparticles
solution due to the inhibition of the enzyme which lost its activity by nanoparticles.
Keywords
Main Subjects
)
