Titanium dioxide nanaoparticles (TiO2 NPs) were prepared using laser ablation technique by ablation of titanium target immersed in distilled de-ionized water (DDW) by Q-switched pulsed Nd:YAG laser of 1064 nm, 1 Hz, (490) mJ and 700 pulse. UV-visible spectrophotometer and Transmission Electron Microscopy (TEM) were used to characterize the optical and morphological properties of prepared nanoparticles, respectively. The absorption spectrum of TiO2 NPs was at ultraviolet-region (214 nm) due to Surface Plasmon Resonance (SPR), and the particles size distribution of the prepared nanoparticles ranged from 30 to 100 nm. The antifungal activity of
TiOR2R NPs was carried out against Microsporum canis. TiOR2R NPs showed
significant inhibitory activity especially at high concentrations and high
exposure times with microbial pathogenesis.