Engineering and Technology Journal

Abstract

Two isolated bacterial strains (E. coli 64, E. coli MM294), were purified,
they are: gram-negative bacteria, circular, regular edged, thick somewhat
glitter, viscous, and lactose fermenter. They belong to the family of
Enterobacteriaceae E. coli 64 showed the resistant to all used antibiotics
except Rifampicin (RA), Cephotaxime (CTX), Nitrofurantion (FT),
Tabramycin (TM), and Cephalexin (RX) (Sensitive to). While E. coli
MM294 showed the sensitivity to all used antibiotics except: Rifampicin
(RA), Lincomycin (L), Clindamycin (CM), and Erythromycin (E),
(resistantt to) Conjugative experiments showed that E. coli strain 64
harbored a self transmissible plasmid carrying resistant genes for:
Tetracycline (TE), Amoxicillin (AMX), Neomycin (N), Chloramphenicol
(C), Ampicillin (AM), and Trimethoprime + Sulfamethorazole (SXT) It
seems that all these resistant genes may be carried on one self transmissible
plasmid, or they may be carried on one self transmissible plasmid (except
Neomycin, resistant gene may carry on other self transmissible plasmid)..