This study was designed for determination of effecting factors on increasing of
enzyme activity (Endo-1-4-β-D- glucanase) isolated from fungi. The stable surface
incubation (SSF) of Aspergillus niger after twelve days gave higher enzymatic
activity of 0.55 unit per mL. After the choosing of optimum circumstances of A.
niger culture on 8th day we found that the highest enzymatic activity 5.20 unit per
mL. And that mean 9th times and reducing 96 hour from operation