Preparation of ELISA Kit for detection of Brucella infection
Engineering and Technology Journal,
2010, Volume 28, Issue 13, Pages 618-626
AbstractPreparation of antigen from Br.melitensis strain Rev1 was done by using
ultrasonication, Column chromatography used to analysis of protein component of
antigen; four peaks on chromatography were observed.
Electrophoresis techniques used for characterization of antigen, different in
molecular weight (M.W) protein bands were showed in electrophoresis ranged
between 478630-123060 Dalton.
A group of guinea pigs was infected experimentally with virulent strain of
Brucella abortus & Brucella melitensis.Peak that obtained by chromatography
were used to indicate Brucella infection with the 2 virulent strains (Brucella
abortus & Brucella melitensis).
The best result obtained by using peak two which have the protein concentration
364.2 μg/ml a comparative study was done using a kit prepared by Synbiotics
Company and the result was positive in Brucella abortus infection and negative in
Brucella melitensis infection.
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