Abstract

The study is aimed to improve of ethanol production from crude whey
dependency on mixed culture of lactose-fermenting yeasts and baker's yeast ( S.cerevisiae) under optimum condition of pH and mixture of sugars concentration, hence studying the effect of defferent agitated speeds on ethanol production. The study was included: Studying the effect of sugar concentration and pH value in working of each yeast
through making expirements for Ethanol and Biomass production from different concentrations whey lactose and experiments, to produce ethanol and Biomass from crude and supplimented whey, by (K2HPO4 NH4Cl, MgSO4.7H2O) by using S.cerevisiae at different pH values, 35 °C for two days . - mix-culture of yeasts above were used for producing ethanol and biomass from different whey Lactose concentrations.
- Studying effects of defferent agitated speeds on ethanol production from whey by the beast co-culture producer , under four Agitated speeds (50, 100, 150, 200) rpm under optimum determined pH5, (6)% lactose concentration ,35°C for two days.
- The results was:
- Obtained two Isolated yeast strains C.kyfer-T, and C.kyfer-Sfrom crude whey.
- Incapable of S.cerevisiae to produce ethanol from all concentrations of synthetic lactose ,crude ,and additive whey lactose , but it able to produce biomass from each type of lactose , the ablity of both C.kyfer strains to produce ethanol and biomass, and C.kyfer –T is the beast producer.
- The beast ethanol production was (5.1)% from only(6)%whey lactose by mixculture of (C.kyfer –T + C.kyfer –S).
- the best was (11.4)% ethanol at (150)rpm , while biomass production was contineous increasing , and the best production was (12.1)% biomass at (200) rpm
by mix-culture of (C.kyfer –T + C.kyfer –S).