A ligand of testosteronthiosemicarbazone was prepared, the chemical
characteristics of this ligand are: yellow powder, soluble in alcohol and its melting point is 100 – 102 °C. The sensitivity of some species of pathogenic bacteria including Escherichia coli, Proteus vulgaris, Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus feacalis to different concentrations of prepared
testosteronthiosemicarbazone (0.05 g/ml, 0.1 g/ml, 0.25 g/ml, 0.5 g/ml, and 1 g/ml) was tested to find out the role of testosteronthiosemicarbazone as an antibacterial agent.
The experimental results of bacterial sensitivity to different concentrations of testosteronthiosemicarbazone, showed a significant sensitivity of all tested bacteria to this ligand at concentration starting from 0.5 g/ml, to 1 g/ml except Pseudomonas aeruginosa, and Streptococcus feacalis which they exhibited their significant sensitivity to the ligand at concentration starting from 0.25 g/ml, and 1 g/ml respectively.