In this study sugar concentration was measured by using diode laser, and compared were blood with those obtained from normal spectroscopic methods were the results laser system was arranged with a by using diode laser with wavelength of 532nm and power of 1 mW was used . While the Values ranged by using spectroscope technique between (70-102mg/dl) ,Wille the measured concentration of blood sugar by laser technique give (69.87-102mg/dl). The standard deviation was calculated and represents the relative comparison between both methods. The comparison results showed that determine the concentration of blood sugar using diode laser technique has a high accuracy and it cost less than that obtained by the spectroscope methods