In this work, zerconia nano particles have been synthesis by using laser ablation in water. AFM measurement displayed that the particle size of Zerconia was 46nm and no other phases have been noticed. The root mean square of surface roughness becomes 0.743nm after laser ablation, while before that was 2.33nm. Granularity distribution chart observed that the particle size distribution of Zerconia before laser ablation was (100-400) nm, while after that become (20-80) nm .The microstructure investigation of zerconia nano particle was carried out by using SEM.