Hasan F.Al-Azzawie
Abstract
The study was investigated the hepatoprotective activity of methanolic extract of Anastatica hierochuntica using carbon tetra chloride (CCl4)-induced hepatotoxicity in rats. The levels ...
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The study was investigated the hepatoprotective activity of methanolic extract of Anastatica hierochuntica using carbon tetra chloride (CCl4)-induced hepatotoxicity in rats. The levels of liver enzymes glutamate oxaloacetate transminase , glutamate pyruvate transminase ,alkaline phosphatase ,total Protein, total and direct bilirubin, in addition to Superoxide dismutase, glutathione peroxidase activities ,glutathione malondialdehyde and total antioxidant status levels were evaluated in experimental rats (with or without CCl4- inducedhepatotoxicity)following intake of 100 mg/kg p.o alcoholic Anastatica hierochuntica extract by using standard procedures compared with standard silymarin at a dose of 100 mg/kg p.o. Results showed that methanolic extract at a dose level of 100 mg/kg had a significant decrease(p<0.05) in activities of serum liver enzymes, glutamate oxaloacetate transminase , glutamate pyruvate transminase, alkaline phosphatase, total bilirubin and protein were significantly decreased in rats treated with CCl4 after 4 weeks compared to that of silymarin group in addition to the methanolic extract had antioxidant activity through decreasing activities of superoxide dismutase and glutathione peroxidase to levels in control rats group. Histopathology of a liver tissue of the animals treated with the extract was also studied to monitor the liver status. The liver biopsy of all experimental rat groups treated with the methanolic Anastatica hierochuntica extract showed significant restoration of the normal histomorphologic pattern of liver cells. From the above results, it is concluded for the first time that methanolic Anastatica hierochuntica extract offers protective effect against CCl4-induced hepatotoxicity in experimental rats.
